Figure 3.

Quantification of heterogeneity of tau-tubulin complexes. Diffusion times of tau-tubulin complexes for constructs based on truncation of 4R and 3R tau are plotted. The lines at 0.60 and 0.67 ms denote the upper (solid) and lower (dashed) boundaries of expected median diffusion times of 1:1 tau-tubulin complexes determined by FCS measurements with excess P1234R′ and P13, respectively, as described in the text. (Inset) The widths of Gaussian fits of each distribution of diffusion times are shown. The lines are full width half-maximum from the measurements corresponding to the lines of median diffusion times in the main plot. Details are in the text and Supporting Material. To see this figure in color, go online.