Table 1.

FCS and Polymerization Parameters for Tau Constructs

Construct		Length	Diffusion Time (ms)		Polymerization (±SD)
			Median	Peak Width	t1/2 (s)	kobs (×10−2 s−1)
4R	P1234R	201	0.87	0.33	32 ± 2	3.1 ± 0.2
			0.67 (excess tau)	0.09
	P1234	179	0.75	0.15	64 ± 12	1.6 ± 0.3
	P123IR3/4	156	0.67	0.12	111 ± 14	0.9 ± 0.1
	P123	143	0.69	0.12	91 ± 9	1.1 ± 0.1
	P12IR2/3	124	0.63	0.10	444 ± 43	0.22 ± 0.02
	P12	112	0.60	0.07	852 ± 86	0.12 ± 0.01
			0.61 (excess tau)	0.08
3R	P134R′	170	0.79	0.23	50 ± 7	2.0 ± 0.3
	P134	148	0.65	0.08	321 ± 17	0.32 ± 0.02
	P13IR3/4	125	0.66	0.09	765 ± 104	0.13 ± 0.02
	P13	112	0.60	0.09	350 ± 44	0.29 ± 0.04
			0.60 (excess tau)	0.07
Scrambled	P1324	179	0.74	0.15	50 ± 6	2.0 ± 0.2
	P1234∗	179	0.76	0.12
	P132	143	0.72	0.15	—	—
	P124	148	0.65	0.09	—	—
Controls	DARPin	169	0.60	0.07
	RB3	147	0.73	0.08
	tubulin	—	0.52	0.08
			0.54 (+20 μM tubulin)	0.08