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. 2017 Jun 20;112(12):2615–2623. doi: 10.1016/j.bpj.2017.05.007

Figure 2.

Figure 2

Extending the neck linker increases the 1HB duration. (A) The sequence of states in the ATP half-site experiment; D∗ denotes a fluorescent nucleotide. (B) Half-site release rates at 2 mM ATP, measured by fitting fluorescence transients (Fig. S4). Open circles represent 9–11 replicates for each motor, with mean value shown as a black bar. (C) Comparison of differences in the 1HB durations (Δτ1HB) between 14- and 17-residue neck-linker constructs for kinesin-1 and kinesin-2 measured by single-molecule (SM) experiments (Fig. 1E) and biochemical (BC) assays (Fig. 2B). Error bars denote propagated error from the SE of short state distributions for single-molecule experiments, and from the SE of half-site values for biochemical assays. To see this figure in color, go online.