Figure 2.

KU-55933 and sorafenib inhibit the proliferation of HepG2, Huh7 and Hep3B cells in a synergistic manner. Cells were seeded in a 24-well plate and were then treated with KU-55933 (10 µmol/L) and sorafenib (5 µmol/L) alone or combination for 3 d. The cell proliferation rate in each well was determined with the CellTiter 96 MTT cell proliferation assay kit (Promega) following the manufacturer's instructions. Columns, mean of absorbance from three separate experiments (*,p<0.05;**, p<0.01; ***, p<0.001);bars , SD.