Table 2.
Primer and probe sequences and their distribution for multiple real-time PCR
| Distribution | Target gene | Primer probe name |
Sequence (5'-3') | Product (bp) |
GenBank No. accession No. |
|---|---|---|---|---|---|
| Reaction 1 | RnaseP | RnaseP-F | 5'-AGATTTGGACCTGCGAGCG-3' | 71 | U77665.1 |
| RnaseP-R | GAGCGGCTGTCTCCACAAGT | ||||
| RnaseP-P | VIC-TTCTGACCTGAAGGCTCTGCGCG-MGB | ||||
| cagH | cagH-F | TTATGTTAGAAATCGCTTGAGTGTCA | 98 | FR666857.1 | |
| cagH-R | CGCTTCTCAAATGATACTTAATCAATC | ||||
| cagH-P | FAM-AGGTGCTAGTAGCTAATC-MGB | ||||
| Reaction 2 | HP23SrRNA | HP23S-F | TTCAGTGAAATTGTAGTGGAGGTG | 98 | NR_076155.1 |
| HP23S-R | TCCCATTAGCAGTGCTAAGTTGTA | ||||
| HP23S-AA | FAM-AGACGGAAAGACC-MGB | ||||
| HP23S-GA | VIC-AGACGGGAAGACC-MGB | ||||
| HP23S-AG | VIC-AGACGGAGAGACC-MGB | ||||
| Reaction 3 | CYP2C19*2 | CY2-F | GCTTGGCATATTGTATCTATACCTT | 85 | NG_008384.2 |
| CY2-R | GATTCTTGGTGTTCTTTTACTTTCT | ||||
| CY2-G | FAM-ATTTCCCGGGAACC-MGB | ||||
| CY2-A | VIC-ATTTCCCAGGAACC-MGB | ||||
| Reaction 4 | CYP2C19*3 | CY3-F | AATTGAATGAAAACATCAGGATTG | 88 | NG_008384.2 |
| CY3-R | ACTGTAAGTGGTTTCTCAGGAAGC | ||||
| CY3-G | FAM-CTGGATCCAGGTAAG-MGB | ||||
| CY3-A | VIC-CCTGAATCCAGGTAAG-MGB |