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. 2017 Jun 20;112(12):2529–2539. doi: 10.1016/j.bpj.2017.03.042

Figure 1.

Figure 1

Amino acid sequence analysis of HsPrp40A. (A) Sequence alignment of CBSs of known centrin targets including HsXPC (UniProt: Q01831), HsSfi1 (UniProt: A8K8P3), HsPOC5 (UniProt: Q8NA72), ScSac3 (UniProt: P46674), and the novel target HsPrp40A (UniProt: O75400). HsSfi1, HsPOC5, and ScSac3 are in a reversed orientation (C- to N-terminal end) to highlight the conserved hydrophobic residues. (B) Helical wheel of the CBS within HsPrp40A comprising the amino acid sequence beginning at Lys524 through Met541. The hydrophobic triad W1L4L8 is identified in gray. The helical wheel representation was generated using an online resource at http://kael.net/helical.htm. (C) Sequence alignment of Prp40A in H. sapiens, M. musculus (UniProt: Q9R1C7), D. renio (UniProt: Q7ZUE4), X. laevis (UniProt: Q08AZ7), and C. reinhardtii (available at the Orthologous Matrix database under accession number CHLRE00747), including ScPrp40 (UniProt: P33203).