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Sequential order of molecular events during the thermal perturbation of the 13C-Crcen-HsPrp40Ap complex (1:1 molar ratio) over the temperature range 5–90°C. The key to complex formation is the inter-molecular salt bridge interactions between HsPrp40Ap’s negatively charged residues and centrin’s Arg residues, as well as the requirement of the hydrophobic interaction within the binding interface. The text in bold corresponds to HsPrp40Ap’s vibrational modes.
