Table 1.
Summary of the Thermodynamic Data for the Interaction between Wild-Type Centrin Homologs and HsPrp40Ap
| Protein | Peptide | Temperature (°C) | Ka (× 106 M−1) | Kd (nM) | ΔGB (kcal/mol) | ΔHB (kcal/mol) | −TΔSB (kcal/mol) | na |
|---|---|---|---|---|---|---|---|---|
| Hscen1 | HsPrp40Ap | 25 | NBb | – | – | – | – | – - |
| Hscen2 | HsPrp40Ap | 25 | 3.6 (0.4) | 278 (31) | −8.95 (0.07) | −15.0 (0.3) | 6.1 (0.3) | 0.481 (0.007) |
| Crcen | HsPrp40Ap | 20 | 2.0 (0.2) | 500 (50) | −8.45 (0.05) | −9.7 (0.1) | 1.2 (0.1) | 1.10 (0.01) |
| Crcen | HsPrp40Ap | 25 | 1.7 (0.2) | 588 (69) | −8.51 (0.07) | −11.2 (0.2) | 2.7 (0.2) | 1.70 (0.02) |
| Crcen | HsPrp40Ap | 30 | 1.3 (0.1) | 769 (59) | −8.48 (0.05) | −13.5 (0.2) | 5.0 (0.2) | 1.6 (0.2) |
| Crcen | HsPrp40Ap | 35 | 2.2 (0.3) | 455 (62) | −8.95 (0.07) | −12.6 (0.2) | 3.6 (0.2) | 1.34 (0.02) |
Numbers in parentheses indicate the mean ± SE.
a
Number of binding sites for HsPrp40Ap.
b
NB, no binding observed.