Table 3.

Mechanical Properties of Model Filaments

Deformation	Actin		Cofilactin
	Wet-Lab	Model	Wet-Lab	Model
Bending	LB = 9.8 ± 0.14 μma	LB = 7.0 ± 0.11 μm	LB = 2.2 ± 0.026 μma	LB = 1.3 ± 0.02 μm
	κ = 39 ± 2.0 × 10−27 N m2a	κ = 29 ± 0.45 × 10−27 N m2	κ = 9.4 ± 2.9 × 10−27 N m2a	κ = 5.3 ± 0.08 × 10−27 N m2
Overtwist, filament subunit	LT,sub = 0.56 ± 0.24 μmb	LT,sub = 0.20 ± 0.01 μm	LT,sub = 0.03 ± 0.01 μmb	LT,sub = 0.03 ± 0.02 μm
	Csub = 2.3 ± 1.0 × 10−27 N m2 rad−1b	Csub = 0.84 ± 0.06 × 10−27 N m2 rad−1	Csub = 0.13 ± 0.06 × 10−27 N m2 rad−1b	Csub = 0.13 ± 0.07 × 10−27 N m2 rad−1
Undertwist, filament subunit	LT,sub = 0.56 ± 0.24 μmb	LT,sub = 0.20 ± 0.01 μm	LT,sub = 0.03 ± 0.01 μmb	LT,sub = 0.03 ± 0.02 μm
	Csub = 2.3 ± 1.0 × 10−27 N m2 rad−1b	Csub = 0.82 ± 0.04 × 10−27 N m2 rad−1	Csub = 0.13 ± 0.06 × 10−27 N m2 rad−1b	Csub = 0.12 ± 0.08 × 10−27 N m2 rad−1
Overtwist, filament end	LT,fil = 6.8 ± 0.7 μmc	LT,fil = 3.2 ± 0.1 μm	NDd	LT,fil = 3.2 ± 0.1 μm
	Cfil = 28 ± 3 × 10−27 N m2 rad−1c	Cfil = 13 ± 0.25 × 10−27 N m2 rad−1		Cfil = 13 ± 0.31 × 10−27 N m2 rad−1
Undertwist, filament end	LT,fil = 6.8 ± 0.7 μmc	LT,fil = 1.2 ± 0.1 μm	NDd	LT,fil = 2.2 ± 0.1 μm
	Cfil = 28 ± 3 × 10−27 N m2 rad−1c	Cfil = 4.9 ± 0.28 × 10−27 N m2 rad−1		Cfil = 8.9 ± 0.30 × 10−27 N m2 rad−1

Bending and torsional persistence length (L_B and L_T) and rigidity (κ and C) values are converted using the following formulas: L_B = κ/k_BT, L_T = C/k_BT. Uncertainties in model measurements represent the standard deviation, N = 5.

^aMcCullough et al. (9).

^bProchniewicz et al. (10).

^cYasuda et al. (44).

^dNot determined.