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. 2017 May 12;8(8):1362–1370. doi: 10.7150/jca.18031

Figure 2.

Figure 2

mTORC1 positively regulates OPN expression. (A) Tca8113 and KB cells were treated with or without 50 nM rapamycin (Rapa) for 24 h. Expression levels of OPN, p-S6 and S6 were detected by western blot analysis (top). The mRNA levels of OPN were analyzed by qRT-PCR (middle). OPN levels in the cell supernatants were detected by an ELISA (bottom). Data indicate mean ± SD of triplicate samples. **P<0.01; ***P<0.001, compared with the respective controls. (B) Tca8113 cells were transfected with mTOR, Raptor or Rictor siRNAs for 48 h. Cell lysates were subjected to immunoblotting with the indicated antibodies (right). The mRNA levels of OPN were analyzed by qRT-PCR (left). Data indicate mean ± SD of triplicate samples. **P<0.01, compared with the respective controls.