Figure 6.

SSBP1 knockdown induced an increased level of DNA damage and impaired the HR repair of DSBs. (a) Confocal microscopy recorded the images in 4 groups as follows: H1299-NC, H1299-sh, H1299-NC-2Gy-1h and H1299-sh-2Gy-1h. (b) The percentages of γH2AX foci-positive cells in these four groups were obtained by analyzing 50 randomly selected cells in each group. (c) The numbers of H2AX foci per cell in these four groups were obtained by analyzing 50 randomly selected cells in each group. (d) The effects of SSBP1 knockdown on double-strand breaks repair proteins (Ku78, Ku80, Rad51, BRCA2, γH2AX), without radiation or 24 h after 6Gy. **P<0.01