Figure 1.

NUP treatment prevented TNFα-induced IκBα degradation and NF-κB nuclear translocation. (A) A549 cells were treated with TNFα (2.5 ng/ml) for 15 min or 1h with or without NUP pretreatment (12 μg/ml for 2h). Cytoplasmic lysates from treated cells were tested for the presence of IκBα by Western blot. (B) A549 cells were treated with TNFα (2.5 ng/ml) for 15 min. with or without NUP pretreatment (12 μg/ml for 2h). Nuclear lysates from treated cells were tested for the presence of NF- κB sub-units (p65 and p50) by Western blot. Anti-β-actin was used as loading control. (C) B16 cells were treated with LPS (1 µg/ml) for indicated times with or without NUP pretreatment* (6 μg/ml for 2h). Nuclear lysates from treated cells were tested for the presence of p65 NF- κB subunit by Western blot. Anti-β-actin was used as loading control. All experiments were repeated at least three times. A representative blot and its densitometry analysis are shown here, normalized to β-actin.