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NUP treatment (6 µg/ml for 2h) activated the ERK signaling pathway. (A) L428 Hodgkin's lymphoma cells (with constitutive NF-κB activation) (B) MCF-7 human breast cancer cells (C) A549 human adenocarcinoma cells (D) B16 murine melanoma cells (E) GA human melanoma cells. Cytoplasmic lysates were prepared and pERK was detected. Anti-ERK or anti-β actin was used as loading control. All experiments were repeated at least three times. Representative blots are shown here.
