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PD98059 ERK inhibitor blocks the effect of NUP on NF-κB translocation. A549 cells were treated with PD98059 (25 μM for 1h) before addition of NUP (6 μg/ml for 2h). Followed by the addition of TNFα (2.5 ng/ml) for the indicated times. The presence of p50 in nuclear extracts and of ERK and pERK in the cells cytoplasmic extracts was detected by Western blot. The experiment was repeated at least three times. A representative blot and its densitometry analysis are shown here.
