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. 2017 Apr 10;7(7):1781–1794. doi: 10.7150/thno.18437

Figure 2.

Figure 2

Fluorescence responses of RhoSSCy to biothiols and pH in vitro. (A) Emission spectra of RhoSSCy under increasing GSH levels (0, 1, 2, 3, 4, 5, 10, 50 and 500 μM). (B) Fluorescence-based GSH titration curve (λex at 480 nm, λem at 580 nm). (C) The linear calibration plot of fluorescent intensity (λex at 480 nm, λem at 580 nm) and its corresponding GSH concentration. (D) Emission spectra of RhoSSCy under decreasing pH value. (E) Fluorescence-based pH titration curve (λex at 640 nm, λem at 765 nm). (F) The pH reversibility study of RhoSSCy between pH 5 and 10.