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In vitro cytotoxicity in brain tumor cell lines. (A) Cytotoxicity assays of U87, GBM43 and U251 cells treated with DOX, DOX-SA-AuNPs, DOX-EGF-AuNPs and DOX-EGF-SA-AuNPs for 72 h. Error bars indicate s.e. (n=6). *P<0.05, **P<0.01 and ***P<0.001 compared with the free-DOX group. (B) Flow cytometric analysis of cleaved caspase-3 in U87 cells treated with 1 μM DOX, DOX-SA-AuNPs, DOX-EGF-AuNPs or DOX-EGF-SA-AuNPs for 72 h; the RL1-A channel is shown.
