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. 2017 Apr 10;7(7):1890–1900. doi: 10.7150/thno.19135

Figure 1.

Figure 1

TAM promotes endometrial cell growth via upregulating SQSTM1 expression. A, MCF7, RL95-2 or AN3CA cells were incubated with various concentrations of 4-OH-TAM for 24 hours. SQSTM1 expression was detected by western blotting. Actin was used as the loading control. B, MCF7, RL95-2 or AN3CA cells were incubated with 4-OH-TAM (15 μM) for 8, 16 or 24 hours. SQSTM1 expression was detected by western blotting. Actin was used as the loading control. Relative SQSTM1 expression (SQSTM1/actin) was quantified and normalized to the relative SQSTM1 expression in the control. C, Various concentrations of 4-OH-TAM were added to MCF7, RL95-2 or AN3CA cells 24 hours after transfection with SQSTM1 siRNAs. MTS assay was performed 72 hours after transfection. The experiments were performed in triplicate and repeated three times. Representative results from one triplicated experiment were shown as Mean±SD. Student's T test was used for the statistical analysis. Asterisks indicate statistical significance. SQSTM1 knockdown was confirmed by Western blotting as in A and B.