Skip to main content
. 2017 Apr 10;7(7):1890–1900. doi: 10.7150/thno.19135

Figure 7.

Figure 7

TAM activated PRKCD to activate Nrf2-SQSTM1 axis. A, Phosphorylation of PRKCD (p-PRKCD) and total PRKCD expression in RL95-2 and AN3CA cell upon 4-OH-TAM treatment at different times were analyzed by western blotting. B, Forty-eight hours after PRKCD siRNAs transfection, 4-OH-TAM (20 μM) was added for another 1 hour. The effect of PRKCD depletion on 4-OH-TAM-induced phosphorylation and expression of Nrf2 were explored by western blotting. The effects of PRKCD depletion on 4-OH-TAM induced SQSTM1 mRNA (C) and protein (D) expression were explored by qRT-PCR or western blotting, respectively. RT-PCR results were shown as Mean±SD. Asterisks indicate statistical significance (Student's T test, p<0.05, n=3). E, Working model: Tamoxifen activated PRKCD to promote Nrf2 S40 phosphorylation. Phosphorylated Nrf2 translocated to the nucleus and stimulate SQSTM1 transcription to promote endometrial hyperplasia, eventually leading to endometrial tumorigenesis.