Figure 3.
miR-708 protected cardiomyocytes against apoptosis induced by hypoxia or isoproterenol. A and B: H9C2 cells were cultured under the hypoxia condition of 1% oxygen for 24 hours with or without overexpression of miR-708, followed by Annexin V analyses (A). Bar graph showing decreased apoptotic cell percentage in the presentence of miR-708 (B). C: Pictures of H9C2 cell with or without overexpression of miR-708 showing different response to 200uM isoproterenol (ISO). Scale bar 50um. D and E: H9C2 cells were treated with 200uM ISO for 24 hours with or without overexpression of miR-708, followed by Annexin V analyses (D). Bar graph showing decreased apoptotic cell percentage in the presentence of miR-708 (E). Data are presented as mean ± SEM (n=3). *p<0.05, **p<0.01.