Figure 4.

miR-708 promoted cardiac regeneration in vivo. A: Schematic representation of the procedure to deliver miR-708 mimics or a negative control using a neutral lipid emulsion (NLE) reagent into the mice with heart injury induced by ISO (n=7 for each group). The effects of miR-708 on the cardiac regeneration and recovery of heart function in vivo were determined. B: Detection of the instant effects of NLE-miR-708 delivery in vivo indicating remarkable increase of miR-708 in the heart, kidney and lung after 3-day's continuous treatment. C: The long-term effects were determined by miR-708 analysis in the hearts at day 16 after the mice were anaesthetized, indicating a little bit higher of the miR-708 levels in the hearts of NLE-miR-708 group compared to controls. D: The echocardiography examinations indicated that ISO treatments exaggerated chamber size and reduced wall thicknesses compared with PBS treated mice, which were rescued by in vivo delivery of miR-708. E: Time course examinations of echocardiography showing the change of EF levels in mice within four weeks after ISO treatment. PBS was used as a negative control (n=5). F: Echocardiography examinations to the mice showing the EF levels decreased from ~65% in PBS group to ~45% in ISO group, and returned to ~55% after 6-day therapy with miR-708 mimics. G: Echocardiography examinations to the mice showing the FS levels decreased from ~35% in PBS group to ~22% in ISO group, and returned to ~30% after treatment with miR-708 mimics. H,I: The increased levels of LVEDV (H) and LVESV (I) were seen in the mice at day 5 and day10 after ISO treatment, which was rescued by in vivo delivery of miR-708. Data are presented as mean ± SEM (n=7). *p<0.05, **p<0.01.