Figure 1.
High glucose promotes the proliferation of breast cancer cells.
Notes: MDAMB231 (A), SKBR3 (B) or MCF-7 (C) cells (1×104) were seeded in 12-well plates and cultured in DMEM supplemented with 2% FBS under low glucose (5 mM) or high glucose (25 mM) condition. The number of cells was counted at the indicated times. (D, E) BrdU incorporation assays were performed on MDAMB231 (D) or SKBR3 (E) cells treated with either DMSO (control), AG1478 (0.25 μM) or PD153035 (0.25 μM) under low glucose (5 mM) or high glucose (25 mM) condition. The percentage of cells incorporating BrdU after 12 hours was shown. (F) MDAMB231 cells treated with either DMSO (control), AG1478 (0.25 μM) or PD153035 (0.25 μM) under high glucose (25 mM) condition (same as the treatment in D). The effects of AG1478 and PD153035 on EGFR phosphorylation were shown.
Abbreviations: DMSO, dimethyl sulfoxide; EGFR, epidermal growth factor receptor; FBS, fetal bovine serum; HG, high glucose; LG, low glucose; WB, western blot.