Figure 3.

High glucose influences c-cbl phosphorylation and GTPase Rac1 activation.

Notes: (A) Serum-deprived MDAMB231 cells were cultured in low glucose condition (5 mM) for 12 hours and then were kept in this condition (control, lane 1) or exposed to high glucose (25 mM) with (5% FBS) or without serum. After 6 hours, these cells were harvested for measurement of c-cbl phosphorylation and total c-cbl levels. The Western blot analysis of c-cbl phosphorylation was quantified and presented by the plot at the bottom. (B, C) Serum-deprived MDAMB231 cells were treated same as (A). Then, these cells were harvested and the activities of Rac1 (B) and Cdc42 (C) were measured by pull-down activity assay. The Western blot analysis of activated Rac1 (B) and activated Cdc42 (C) were quantified and presented by the plots at the bottom.

Abbreviations: FBS, fetal bovine serum; HG, high glucose; LG, low glucose; WB, western blot; WCL, whole cell lysates.