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. 2017 Jun 13;9:429–436. doi: 10.2147/BCTT.S135665

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© 2017 Hou et al. This work is published and licensed by Dove Medical Press Limited

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High glucose-induced signaling regulates the proliferation of breast cancer cells.

Notes: Cdc42 knockdown MDAMB231 cells (A), Rac1 knockdown MDAMB231 cells (B), MDAMB231 cells expressing HA-tagged Cdc42 (F28L) (C), or MDAMB231 cells expressing HA-tagged Rac1(G12V) (D) were seeded (1×10⁴) in 12-well plates and cultured in DMEM supplemented with 2% FBS under low glucose (5 mM) or high glucose (25 mM) condition. The number of cells was counted at the indicated time points. (E) BrdU incorporation assays were performed on MDAMB231 cells expressing vector control (columns 1 and 4), HA-tagged Rac1 (G12V) (column 2), or HA-tagged Cdc42 (F28L) (column 5), which were cultured under low glucose (5 mM) or high glucose (25 mM, columns 3 and 6) condition. The percentage of cells incorporating BrdU after 12 hours was shown.

Abbreviations: FBS, fetal bovine serum; HG, high glucose; LG, low glucose.