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. Author manuscript; available in PMC: 2018 Mar 14.
Published in final edited form as: Cell Rep. 2017 Mar 14;18(11):2622–2634. doi: 10.1016/j.celrep.2017.02.059

Figure 2. Knocking Down METTL3 Expression Promotes the Growth and Self-Renewal of GSCs.

Figure 2

(A) RT-PCR analysis of METTL3 expression in GSCs transduced with lentivirus expressing control shRNA (shC) or METTL3 shRNAs (shMETTL3-1, shMETTL3-2). n = 3. See also Figure S1.

(B–D) Cell growth (B), sphere-formation (C), and limiting dilution assay (LDA) (D) of GSCs transduced with lentivirus expressing control shRNA or METTL3 shRNAs. A sphere-formation assay and LDA were used to evaluate the self-renewal capacity of GSCs. n = 4 for (B), n = 6 for (C), and n = 20 for (D).

*p < 0.05, **p < 0.01, and ***p < 0.001 by Student's t test. Error bars represent SD of the mean.