. Author manuscript; available in PMC: 2018 May 5.

Published in final edited form as: J Mol Biol. 2017 Mar 31;429(9):1396–1405. doi: 10.1016/j.jmb.2017.03.027

Table 1.

Kinetic analyses of MDHs and acetylated variants^a

	k_cat (s⁻¹)	K_M_{, NAD}⁺(mM)	K_M_{, malate} (mM)	k_cat/K_M_{, NAD}⁺ (S⁻¹ mM⁻¹)	k_cat/K_M_{, malate} (S⁻¹ mM⁻¹)
eMDH WT	20.2 ± 0.4	0.23 ± 0.02	2.61 ± 0.20	87.8	7.74
eMDH 99AcK	51.2 ± 1.3	0.25 ± 0.07	2.32 ± 0.32	204.8	22.07
eMDH 140AcK	74.3 ± 1.1	0.26 ± 0.06	2.54 ± 0.64	285.8	29.25
hMDH2 WT	69.6 ± 2.9	0.16 ± 0.01	0.24 ± 0.08	435.0	290.0
hMDH2 307AcK	178.9 ± 3.1	0.12 ± 0.04	0.20 ± 0.12	1490.8	894.5

The mean values and standard errors were calculated based on three replicates. The parameters were determined by nonlinear regression with software GraFit (Erithacus Software).