Fig 1. SensiScreen^® assay design.

SensiScreen^® KRAS exon 2 features 7 mutation-specific assays as well as a common reference assay. All assays include a common reverse primer and a green fluorescent HydrolEasy™ probe. The reference assay features an allele-independent forward primer (not shown), while the mutation-specific assays include allele-specific forward primers and a BaseBlocker™. The allele-specific forward primers include the particular sequence variation in the 3´-end (green) and can include an additional sequence variation in position +3 or +4 relative to the 3´-end (purple). The BaseBlocker™ is complementary to the wild type sequence (yellow) and specifically blocks amplification of the wild-type allele.