Fig 4. Rotor-Gene 6000 PCR amplification plots of SensiScreen^® KRAS exon 2 multiplex assays using serial dilutions of mutated DNA in a wild type background.

50 ng and/or approximately 16,000 copies of DNA was added to each reaction. The threshold was set at 10% of the average fluorescence signal of the reference assay at cycle 45. Legend describes the fraction of cell line DNA and/or mutated copies of KRAS exon 2 templates.