Table 1. Infection of mice with L- and S-type prions with or without passaging in GT1-7 cells.
| Inoculum 1 | PrPSc in inoculum 2 | Subsequent passages in mice | |||
|---|---|---|---|---|---|
| n/n0 3 | Survival days 4 | Prion phenotype | |||
| Exp. 1 6 | Mo1 brain (463 days, L-type) 5 | + | 8/9 | 196 ± 31.6 | S-type |
| 1/9 | 289 | L-type | |||
| GT1-7 exposed to Mo1 brain | + | 9/9 | 278 ± 5.7 | L-type | |
| Mo2 brain (251 days, S-type) | + | 10/10 | 148 ± 8.9 | S-type | |
| GT1-7 exposed to Mo2 | – | 8/9 | 288 ± 36.8 | S-type | |
| 1/9 | 375 | L-type | |||
| Mo3 brain (150 days, S-type) | + | 5/5 | 143 ± 4.3 | S-type | |
| GT1-7 exposed to Mo3 brain | – | 9/9 | 246 ±17.6 | S-type | |
| Exp. 2 | Mo1’ brain (469 days, L-type) | + | 5/10 | 220 ± 7.2 | S-type |
| 5/10 | 290 ± 6.4 | L-type | |||
| GT1-7 exposed to Mo1’ brain | + | 9/9 | 285 ± 15.4 | L-type | |
| Mo2’ brain (295 days, L-type) | + | 1/11 | 195 | S-type | |
| 10/11 | 273 ±13.8 | L-type | |||
| GT1-7 exposed to Mo2’ brain | + | 9/9 | 277±14.7 | L-type | |
| Mo3’ brain (276 days, L-type) | + | 5/5 | 191 ± 7.1 | S-type | |
| GT1-7 exposed to Mo3’ brain | + | 10/10 | 280 ± 13.1 | L-type | |
1 Inoculum (brain or GT1-7 cells) was intracerebrally injected into ICR mice.
2 PrPSc accumulation was confirmed by western blotting: +, PrPSc positive;–, PrPSc negative.
3 Number of diseased mice out of total number of challenged mice.
4 Survival periods were determined as the time from inoculation to the clinical endpoint or death (mean survival days ± standard deviation).
5 Survival periods and prion phenotype of the individual mouse used for the inoculation are indicated.
6 Experiment numbers are the same as in Fig 1.