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. 2017 Jun 21;12(6):e0179665. doi: 10.1371/journal.pone.0179665

Table 4. Digestible Lys requirements from 1 to 12 d of age (Experiment 1).

Model Response variable Equation Estimated requirement (100, 99, 95%) P-value R2
Quadratic polynomial1 BW gain y = -882.2 + 1972.3x – 776.2x2 1.271, 1.258, 1.207 0.0001 0.8404
FCR5 y = 5.022–6.156x + 2.457x2 1.253, 1.240, 1.190 0.0001 0.7135
Linear broken-line2 BW gain y = 363.6–568.0 × (1.090 –x) 1.090, 1.079, 1.036 0.0001 0.8732
FCR y = 1.189 + 1.696 × (1.081 –x) 1.081, 1.070, 1.027 0.0001 0.7428
Quadratic broken-line3 BW gain y = 364.6–1685.8 × (1.172 –x)2 1.172, 1.160, 1.113 0.0001 0.8765
FCR y = 1.187 + 5.315 × (1.158 –x)2 1.158, 1.146, 1.100 0.0001 0.7441
Exponential asymptotic4 BW gain Y = 261 + 106 × (1 –EXP(– 12.829 × (X– 0.97))) –, 1.329, 1.204 0.0001 0.8743
FCR Y = 1.524–342 × (1 –EXP(– 14.825 × (X– 0.97))) –, 1.281, 1.172 0.0001 0.7437

1 Quadratic polynomial regression model (QP): Y = β0 + β1 × X + β2 × X2, where Y is the dependent variable, X is the dietary Lys concentration, and β0 is the intercept, β1 and β2 are the linear and quadratic coefficients, respectively; maximum response concentration was obtained by:—β1 ÷ (2 × β2).

2 Linear broken-line model (LBL): Y = β0 + β1 × (β2—X), where (β2—X) = 0 for X > β2, Y is the dependent variable, X is the dietary Lys concentration, β0 is the value at the plateau, β1 is the slope and β2 is the Lys concentration at the break point.

3 Quadratic broken-line model (QBL): Y = β0 + β1 × (β2—X)2, where (β2—X) = 0 for X > β2, Y is the dependent variable, X is the dietary Lys concentration, β0 is the value at the plateau, β1 is the slope and β2 is the Lys concentration at the break point.

4 Exponential asymptotic (EA): Y = β0 + β1 × (1 –EXP(– β2 × (X– β3), where Y is the dependent variable, X is the dietary Lys concentration, β0 is the response for the dependent variable estimated for the feed with the lower Lys, β1 is the difference estimated between the minimum and maximum response obtained by the increasing Lys, β2 is the slope of the exponential curve, β3 is the Lys at the lower level; requirement were estimated by calculating (ln(0.05)/– β2) + β3 for 95% of the requirement and (ln(0.01)/– β2) + β3 for 99%.

5 Feed conversion ratio corrected for mortality weight.