Fig 2. Genes differentially expressed by FCRL4⁺ and FCRL4^- cells at D4 of culture.

MBCs from three healthy donors were activated with sCD40L and ODN with cytokines for 4 days. At D4, FCRL4⁺ and FCRL4^- cells were cell-sorted by flow cytometry and RNA was extracted and hybridized to GeneChip® human genome U133 Plus 2.0 microarrays. Genes differentially expressed in the two cell populations were identified using the SAM statistical method (P < 0.05; fold change ≥ 2 and false decovery rate ≤ 0.01). (A) Genes encoding cell surface markers, chemokine receptors and transcription factors. (B) Genes encoding cell cycle proteins. (C) Genes encoding ITIM-containing receptors and downstream signaling molecules.