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. Author manuscript; available in PMC: 2017 Jun 21.
Published in final edited form as: Biochemistry. 2016 Apr 4;55(15):2197–2213. doi: 10.1021/acs.biochem.5b01354

Figure 8.

Figure 8

Correlation of CD4-binding site antibody binding and local structural dynamics. (A) Peptides detected in H/D exchange, which cover the critical interface residues on the gp120 monomer (PDB entry 4NCO) upon binding of CD4bs antibodies, are colored. (B–D) Plots of deuterium exchanges at a single time point, where the largest differences were observed for the four gp120s, of different peptides covering the binding interface of CD4bs-binding antibodies vs KD (left) or on-rate (right) with linear regression. Data for FL, ΔV3, ΔV1/V2, and Coree are shown as triangles, squares, circles, and diamonds, respectively. Error bars represent standard deviations for two independent measurements.