. Author manuscript; available in PMC: 2017 Jun 21.

Published in final edited form as: Biochemistry. 2016 Apr 4;55(15):2197–2213. doi: 10.1021/acs.biochem.5b01354

Table 1.

Dimensions of Molecules Derived from SAXS and SLS

sample	R_g (Å)		D_max^a	Porod volume (Å³)^b	mass (kDa)
sample	Guinier	GNOM^a	D_max^a	Porod volume (Å³)^b	SLS^c	expected^d
FL	36.5 ± 0.09	37.1 ± 0.04	127.9	222581	93.735	96.046
ΔV3	35.9 ± 0.03	37.1 ± 0.06	125.7	201932	89.969	88.485
ΔV1/V2	32.6 ± 0.03	32.0 ± 0.04	113.9	157757	70.995	72.563
Core_e	32.4 ± 0.07	33.5 ± 0.05	113.7	151814	69.528	68.709

Real space R_g and D_max calculated from GNOM.⁵⁰

Porod volumes determined by Autoporod.⁵⁷

Molecular mass determined by static light scattering (SLS). Uncertainty is largely dependent on the accuracy of the concentration determination, typically ±10%.

The expected molecular mass was based on the predominant glycoforms observed during H/D-exchange MS analysis.