Table 2.
Octet Biolayer Interferometry Binding Parametersa for gp120–Antibody Interactions with CD4i-Binding Antibodiesb
| antibody | parameter | FL | ΔV3 | ΔV1/V2 | Coree |
|---|---|---|---|---|---|
| 17b | ka (M−1 s−1) | 2.5(1) × 104 | 8.1(5) × 104 | 6(1) × 105 | 1.34(5) × 106 |
| kd (s−1) | 8(1) × 10−5 | 1.4(2) × 10−4 | 9.7(2) × 10−5 | 3.5(9) × 10−5 | |
| KD (nM) | 3.0(3) | 1.73(8) | 1.6(3) × 10−1 | 2.6(8) × 10−2 | |
| 48d | ka (M−1 s−1) | 4.8(4) × 104 | 8.9(1) × 104 | 6.6(5) × 105 | 1.6(1) × 106 |
| kd (s−1) | 1.54(1) × 10−4 | 8.9(3) × 10−5 | 1.5(5) × 10−4 | 3.8(3) × 10−4 | |
| KD (nM) | 3.2(3) | 1.01(5) | 2.4(9) × 10−1 | 2.42(7) × 10−1 | |
| A32 | ka (M−1 s−1) | 1.12(1) × 105 | 2.69(8) × 105 | 6.4(2) × 105 | 8.220(3) × 105 |
| kd (s−1) | 1.72(6) × 10−5 | 7.04(3) × 10−5 | 4.8(3) × 10−5 | 4(1) × 10−5 | |
| KD (nM) | 1.54(5) × 10−1 | 2.62(7) × 10−1 | 7.5(7) × 10−2 | 4.1(9) × 10−2 | |
| N5i5 | ka (M−1 s−1) | 7.7(4) × 104 | 1.97(9) × 105 | 6.4(6) × 105 | 1.11(3) × 106 |
| kd (s−1) | ndc | ndc | ndc | ndc | |
| KD (nM) | ndc | ndc | ndc | ndc |
a
ka, rate of association; kd, rate of dissociation; KD, equilibrium dissociation constant.
b
Values are means ± standard deviations. Standard errors are for two independent measurements, presented in parentheses with respect to the smallest number place value: 2.4(1) × 104 is equivalent to 24000 ± 1000.
c
Because of slow dissociation, dissociation rates or binding constants could not be reliably fit.