Table 3.
Octet Biolayer Interferometry Binding Parametersa for gp120–Antibody Interactions with CD4-IgG2- and CD4bs-Binding Antibodiesb
| antibody | parameter | FL | ΔV3 | ΔV1/V2 | Coree |
|---|---|---|---|---|---|
| CD4-IgG2 | ka (M−1 s−1) | 1.2(1) × 105 | 1.6(2) × 105 | 1.780(7) × 105 | 4.74(6) × 105 |
| kd (s−1) | 1.5(4) × 10−4 | 1.5(3) × 10−4 | 5.4(3) × 10−5 | 5.9(8) × 10−5 | |
| KD (nM) | 1.3(2) | 9.1(8) × 10−1 | 3.4(7) × 10−1 | 1.2(1) × 10−1 | |
| NIH45−46 | ka (M−1 s−1) | 5(1) × 104 | 9(2) × 104 | 2.5(1) × 105 | 3.94(2) × 105 |
| kd (s−1) | 1.8(6) × 10−4 | 2.6(3) × 10−4 | 8.2(4) × 10−5 | 9(1) × 10−5 | |
| KD (nM) | 3.4(3) | 2.8(4) | 3.3(4) × 10−1 | 2.4(3) × 10−1 | |
| VRC01 | ka (M−1 s−1) | 3.8(3) × 104 | 5.4(1) × 104 | 3.0(9) × 105 | 5.9(2) × 105 |
| kd (s−1) | 1.7(3) × 10−4 | 1.1(2) × 10−4 | 6.4(3) × 10−5 | 10(2) × 10−5 | |
| KD (nM) | 4.4(4) | 1.9(3) | 2.1(3) × 10−1 | 1.59(4) × 10−1 | |
| VRC03 | ka (M−1 s−1) | 6.5(6) × 103 | 6.7(9) × 103 | 6(2) × 104 | 2.2(8) × 105 |
| kd (s−1) | 1.8(3) × 10−3 | 5(1) × 10−3 | 4.5(3) × 10−4 | 1.41(3) × 10−3 | |
| KD (nM) | 275(16) | 803(75) | 8(3) | 7(2) | |
| F105 | ka (M−1 s−1) | 1.5(4) × 105 | 2.5(2) × 105 | 1.4(2) × 105 | 2.9(2) × 105 |
| kd (s−1) | 8.7(8) × 10−4 | 1.05(1) × 10−3 | 4.6(4) × 10−4 | 4.4(4) × 10−4 | |
| KD (nM) | 5.9(9) | 4.3(3) | 3.4(3) | 1.50(4) | |
| b12 | ka (M−1 s−1) | 2.2(5) × 105 | 4.5(5) × 105 | 2.7(5) × 105 | 1.1(1) × 106 |
| kd (s−1) | 1.960(7) × 10−3 | 2.66(5) × 10−3 | 4.9(9) × 10−3 | 4.8(3) × 10−3 | |
| KD (nM) | 9(2) | 5.9(7) | 17.6(8) | 4.5(2) |
a
ka, rate of association; kd, rate of dissociation; KD, equilibrium dissociation constant.
b
Values are means ± standard deviations. Standard errors are for two independent measurements, presented in parentheses with respect to the smallest number place value.