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. Author manuscript; available in PMC: 2018 May 23.
Published in final edited form as: Biochemistry. 2017 Apr 14;56(20):2584–2593. doi: 10.1021/acs.biochem.7b00078

Figure 2.

Figure 2

(A) MS-MS spectrum of the C-terminus of PTHR. The peak heights show the relative abundances of the corresponding fragmentation ions, with the annotation of the identified matched amino terminus-containing b ions in blue and the C-terminus-containing y ions in red. (B) In FP competition assays a mixture of FITC-PTHRct-9 (0.5 μM) and PDZ1 (7 μM) was incubated with increasing concentrations of the unlabeled PTHRct-9, Cys-PTHRct-9 or PTHR-pThrct-9 (competitors). (C) PTHR-Cys591 exhibited similar cAMP formation in response to challenge with PTH(1–34) as wild-type PTHR.