Table 2.

Binding Affinity of PDZ domains for C-terminal PTHR peptides determined by FP, ITC, and SPR

PDZ	FPa		ITCb		SPRa
	Ki μM	ΔG° kcal/mol	KDμM	ΔG° kcal/mol	KDμM	ΔG° kcal/mol
PDZ1	nm	nm	11.7 ± 0.8	−6.7 ± 0.2	16.0 ± 0.5	−6.3 t 0.0
PDZ2	7.0 ± 0.5	−7.0 ± 0.1	9.7 ± 1.1	−6.9 ± 0.4	11.5 t 0.4	−6.5± 0.0

^ab-PTHRct-22;

^b-PTHRct-8; nm – not measured. The K_D and K_i represent the mean ± S.E.M. (n = 3)