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. 2017 Jun 21;12(6):e0179772. doi: 10.1371/journal.pone.0179772

Fig 4. miR-144-3p inhibited autophagosome formation.

Fig 4

(A and B) Transmission electron microscopy confirms repression of autophagy by miR-144-3p overexpression or knockdown. RAW264.7 cells were transfected with miR-144-3p control, miR-144-3p mimic or miR-144-3p inhibitor for 24 h, and then infected with BCG for 24 h. M, mitochondrion. Autophagosomes (denoted by red arrows). The number of autophagosomes per cross-sectioned cell was counted (15 cells per group counted by TEM). *p<0.05, **p<0.01. (C and D) Overexpression of miR-144-3p could attenuate induction of autophagy by rapamycin. The BCG-challenged RAW264.7 cells were transfected with miR-144-3p mimics, and then treated with 50 μg/ml rapamycin for 2 h. Meanwhile the BCG-challenged RAW264.7 cells were treated with rapamycin or 0.1% DMSO as controls. BCG bacteria (denoted by black arrows). M, mitochondrion. Autophagosomes (denoted by red arrows). The number of autophagosomes per cross-sectioned cell was counted (15 cells per group counted by TEM). **p<0.01, ***p<0.001.