Figure 6.

Colocalization of CLIC5A and transferrin receptors. pLENTI control and CLIC5A overexpressing Reh cells were used for colocalization studies. Immunostaining of both CLIC5A and transferrin receptor were performed in the same cells simultaneously with Hoechst DNA staining. Results were obtained at 100× magnification (upper panels; scale bar = 10μm). A strong colocalization was observed between CLIC5A and transferrin receptors. Additional enlargement for the marked region of the initial image is presented in lower panels (scale bar = 2μm). A merged image was generated (right panels).