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oPACE facilitates endosomal escape of genes. U87MG cells were transfected with FITC-Cy5 double labelled DNA (pLuc) by PACE or oPACE9-20. (A) Standard curves between FITC/Cy5 fluorescence ratio (measured by FACS) and intracellular pH of cells. (B) The FITC/Cy5 ratio of the transfected U87MG cells was measured and converted into pH values using the standard curves. Error bars indicate standard deviation (n=3, ****: p<0.0001).
