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. 2017 Jun 21;3(6):e1700090. doi: 10.1126/sciadv.1700090

Fig. 3. Ipafricept induces mitotic catastrophe when dosed sequentially before paclitaxel.

Fig. 3

Administration of vantictumab or ipafricept 2 days before paclitaxel is essential for blocking tumor growth. (A) UM-PE13 treated with vantictumab (25 mg/kg) on day 1 or 4 with paclitaxel (pac; 20 mg/kg) given on day 1 or 4 in 3-week cycles (n = 6 to 8 per group). (B) OMP-OV38 treated with ipafricept (25 mg/kg) on day 1 or 3 with paclitaxel (20 mg/kg) on day 1 or 3 in 2-week cycles (n = 9 per group). (C) OMP-OV19 treated with ipafricept (25 mg/kg) on day 1 and paclitaxel (20 mg/kg) on day 1 or 3 in 2-week cycles (n = 8 to 10 per group). *P < 0.01; **P < 0.001 combination versus chemotherapy. Data are means + SEM. (D) Tumors from OMP-OV38 as shown in (B) from study day 51 were processed as FFPE with IHC for pHH3 and β-catenin. β-Catenin was detected with HRP-DAB, and pHH3 was detected with AP–Warp Red, with hematoxylin counterstain (magnification, ×20). (E) Tumors from OMP-OV38 as shown in (B) from study day 51 were processed to RNA, and quantitative real-time polymerase chain reaction (PCR) was performed. Gene expression was normalized to control group using the relative quantification method. Data are means + SEM, four biological replicates per group; *P < 0.05, significant compared to paclitaxel.