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. Author manuscript; available in PMC: 2017 Jun 21.
Published in final edited form as: Nature. 2016 Sep 19;539(7629):437–442. doi: 10.1038/nature19834

Extended Data Figure 5. Effect of PI3Kγ inhibition on murine and human macrophage polarization.

Extended Data Figure 5

(a) Relative mRNA expression of immune response transcripts in IL-4 and IFNγ/LPS stimulated vehicle and PI3Kγ inhibitor (IPI-549) treated (a) murine and (b) human macrophages (n=3, p=0.001). (c) Relative mRNA expression of M2 macrophage markers (Arg1, Fizz1 and Ym1) in WT and p110γ−/− IL4-stimulated macrophages (n=3). (d) Relative expression of MHC family members in WT and p110γ−/− IL4-stimulated macrophages (n=3). (e–f) Time course of cytokine mRNA expression in IFNγ/LPS, LPS and IL-4 stimulated (e) WT vs p110γ−/− and (f) vehicle vs. PI3Kγ inhibitor (IPI-549)-treated macrophages (n=3). (g) Relative mRNA expression in mCSF-stimulated WT vs p110γ−/− and IPI-549- vs vehicle-treated macrophages (n=3). (g) Relative nuclear RelA DNA binding activity in IFNγ/LPS stimulated WT and p110γ−/− macrophages (n=3).