Author response image 1. Devices loaded with the indicated siRNAs were implanted into MDA-MB-453 xenografts of un-dosed mice or mice that were pre-dosed with GDC0941 (75mg/kg) for 5 days.
Two days later, the devices were removed, xenograft tissue surrounding each reservoir was stained for Hematoxylin and Eosin., and density of intact nuclei was quantified.% reduction in intact nuclei upon PI3K inhibition is presented. Shown is the mean and SD of at least 4 replicates. B. Proportional effects of the indicated siRNAs with (red) or without (blue) PI3K inhibition as compared to the effect of siGFP in non-treated mice. Bars indicate SD from at least 4 replicates. The expected proportional effect for treatment combination (grey) was calculated according to the Bliss independence model using single treatment effects.