Water radial-arm maze flexibility task performance. (a) From the last day of RT9 to the first day of the flexibility task with two flipped platform locations, all subjects had impaired performance on the first day of exposure to the two flipped spatial locations, regardless of age or follicular depletion status. (b) No differences in performance were noted within the Young cohort across days, regardless of follicular depletion status. (c) Within the Middle-Aged cohort, on the final day of the flexibility task, there was a marginal main effect of Follicular Depletion, where VCD-treated animals made more errors than Vehicle-treated counterparts. (d) No differences in performance were noted within the Vehicle cohort across days, regardless of age. (e) Within the VCD cohort, on the final day of the flexibility task, there was a marginal main effect of Age, where older animals made more errors than younger animals. (# = p<0.10).