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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Toxicol In Vitro. 2017 Mar 9;41:223–231. doi: 10.1016/j.tiv.2017.03.002

Figure 2. Evaluation of uncoated or coated MWCNT uptake in HBMECs.

Figure 2

HBMECs were plated on plastic to form monolayers and treated the following day with cut uncoated 3H-MWCNT, cut DSPE-PEG coated 3H-MWCNT, or vehicle for the specified times. (A) Cells were washed twice in ice cold PBS, harvested in lysis buffer, and 3H activity was assessed using a scintillation counter. Percent uptake was calculated based on input decays per minute. Three samples were used for each condition and the data are displayed as the mean ± standard error of each measurement. (B) Images taken after 24 h treatment show increased aggregation and sedimentation of uncoated MWCNTs on to HBMECs. By comparison, no aggregated or sedimented cut, DSPE-PEG coated MWCNTs are apparent. *p<0.05 (T-Test).