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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Mol Carcinog. 2017 Mar 6;56(7):1789–1797. doi: 10.1002/mc.22636

Figure 5.

Figure 5

The effect of PHB knockdown on Akt kinase activity as well as cell survival and proliferation upon UVB irradiation. HaCaT cells were stably transfected with scrambled shRNA (control) or PHB shRNA (PHB knockdown). (a) PHB expression in the cells was examined by western blotting analysis. The data represents three experiments. (b) Phospho-Akt in the cells treated or not treated with UVB (50 mJ/cm2, 6 h) was immunoprecipitated by immoblilized p-Akt primary antibody and incubated with GSK 3 fusion protein. Then the phosphorylation of GSK-3α/β was detected by western blotting analysis. The data represents three experiments. (c) Survival and proliferation of the cells treated or not treated with UVB (5 mJ/cm2) were assessed by clonogenic assay. The data represents the means ± SD of three independent experiments.