Figure 3. Increased accumulation of Ly6C⁺ leukocytes in lungs in mice pretreated with BKM120.

(A) Significantly increased lung accumulation of CD11b⁺Ly6C⁺Ly6G^low leukocytes in the mice pretreated with BKM120. Non-tumor bearing BALB/c mice (9 week-old females) were treated with vehicle or BKM120 (30 mg/kg, daily) for two weeks, mice were sacrificed then single cell suspensions were generated from lungs and FACS analysis was performed. Plots are gated for CD45⁺CD11b⁺ cells. No differences were found in T cell number (plots are gated for CD45⁺CD3⁺ cells). (B) Gene expression profile (quantitative real-time PCR) FACS sorted CD11b⁺Ly6C⁺Ly6G^low leukocytes from lung of mice treated with BKM120 as described above in A, * indicates p<0.05. (C) MHCII expression on CD11b⁺Ly6C⁺Ly6G^low leukocytes in lungs. (D) The in vitro differentiation of myeloid cells from mouse bone marrow (top) and human bone marrow (bottom) in presence of GM-CSF, 20 ng/ml and BKM120, 300 nM.