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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Clin Cancer Res. 2016 Dec 21;23(13):3371–3384. doi: 10.1158/1078-0432.CCR-16-2142

Figure 6. Effect of loss of PI3Kγ on MMTV-PyMT tumor growth, but not metastasis, is partially recovered by depletion of CD8+ T cells.

Figure 6

(A) Tumor bearing mice (n = 5 per group, 3 X 105 cells injected) were given 100µg of anti-CD8 or isotype control IgG for 3 days, then every third day for 3 weeks to deplete CD8+ T cells at which time mice were euthanized then lung and splenic CD4+ and CD8+ T cell content was analyzed by FACS. Shown here are representative FACS analysis plots from one mouse. The mean CD4 and CD8 ±SEM from each treatment group is shown in graph format as insert on the right. The p-values were determined by Wilcoxon rank sum test. (B) Tumor growth over 9 weeks of WT or PI3Kγ−/− mice treated with control IgG or anti-CD8. Statistics: two-way ANOVA. (C) Number of lung metastasis in WT or PI3Kγ−/− mice treated with or without CD8 T cell depletion. Statistics: two sample t-test. *p < 0.05, **p< 0.01, ***p< 0.001, ****p < 0.0001 and NS = not-significantly different.