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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: Ann N Y Acad Sci. 2017 Apr 26;1397(1):119–129. doi: 10.1111/nyas.13342

Figure 3.

Figure 3

Normal human claudin-5 is 218 amino acids regardless of genotype. (A) Claudin-5 immunoblot showing (1) HeLa cells transfected with untagged human claudin-5 (303), (2) HeLa cells transfected with untagged human claudin-5 (218), (3) human lung tissue, and (4) Caco2 cells. (B) Immunoblot of HeLa cells transfected with either the 303– or 218–amino acid form of claudin-5. Lanes 1–4 correspond to total lung samples from different donors with different genotypes. (C) DNA isolated from the samples in B was amplified by PCR and analyzed for AvrII cleavage as described in the methods section. Genotypes for the lanes in B were 1: G/A, 2: G/G, 3: G/A; and 4: A/A. In all cases, only the 218–amino acid form of claudin-5 was detected.