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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Mol Carcinog. 2017 Mar 6;56(7):1733–1742. doi: 10.1002/mc.22630

Figure 6.

Figure 6

Effects of p120-catenin isoforms in human epidermoid carcinoma cells. (A) Transient transfection increased the levels of p120-catenin isoforms 1A and 4A (hatched boxes). Immunoblotting did not reveal a corresponding increase in p120-catenin isoform 3A (oval), possibly due to the high endogenous levels of this isoform in A431NS cells. Effects of p120-catenin isoforms were examined on (B) cell viability; at the 96-h time-point, p120-catenin isoforms 1A, 3A, and 4A were significantly different from the vector control (p<0.05). (C) Bromodeoxyuridine (BrdU) incorporation, mean±SD, n=3; insert shows BrdU-positive cells, white arrows; (D) cell invasion assays, using real-time monitoring as reported by Parasramka et al. [29]; representative findings from at least three replicate experiments, each giving similar outcomes. The slope of the line for p120-catenin isoform 1A was significantly different from the other slopes shown, including the vector control, p<0.05.