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. 2017 Jun 21;7:3978. doi: 10.1038/s41598-017-04432-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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U94A expression inhibits hOPC migration in vitro. (a) Nested rtPCR shows that hOPCs infected with HHV-6A express U94A mRNA at 4 and 10 days post-infection. (b) Lentiviral expression of U94A RFP significantly inhibits hOPC migration as analyzed by an agarose drop assay. (c) U94A RFP expression does not affect hOPC proliferation over the course of 21 days in vitro. (d) Proportion of dividing hOPCs, determined by BrdU incorporation after 4, 24, and 48 hours of incubation. (e,f) U94A RFP expression does not significantly affect OPC differentiation into GFAP+ astrocytes or GalC+ oligodendrocytes in vitro. Data from 3–6 independent experiments, normalized to GFP+ controls, pooled and displayed as mean ± standard error of the mean (SEM); ns = not significant; ***p < 0.001 versus control. Effects of U94A expression on hOPC proliferation and BrdU incorporation were analyzed by two-way ANOVA. Effects of U94A expression on hOPC migration and differentiation were analyzed by unpaired student’s t-test. Nested rtPCR agarose gel shown as cropped image. Full images shown in supplementary information.